moist and nutrient-rich conditions are more favorable for non-lichenized generalists (cf. Moreover, open wall joints in area B provide numerous small niches for depositions of soil, moisture, bird droppings and other nutrient sources that may influence microbial growth. The suspension was incubated for 4 h at 65C. Even after this treatment, the original shape of the cell wall was still preserved (Fig. Lichen alga were found in samples from both walls, but just in low numbers. The isolation procedure of melanins from cell walls of Phaeococcomyces illustrates the rigidity of the melanised cell wall.
Burg gleichen göttingen
With respect to species richness different intensities of bead beating were applied on two samples. The walls are approximately 400 nm in thickness (Fig. Since the filamentous fungi are endolithic, these particles are not just attached to the building surface, but are deposited in deeper layers of the material. Electron microscopy, for electron microscopy, vegetative filaments were either taken from solid or liquid cultures, concentrated by filtering over.45-m size pore filter and embedded in resin according to Spurr ( 1969 ).
Louis, MO, USA) and for 2 h in osmium tetroxide (1, w / v, aqueous solution, Science Services, Munich). For some growth experiments, a piece of Seeberger Sandstein was grounded in a mortar and sterilized by autoclaving. Most of the ascomycetes, either identified by their sequences or isolated from the stone surface are known as melanised strains. Several ascomycetous black fungi could be isolated from the samples, especially from area B, as listed in Table. In addition, numerous Sarcinomyces and Phaeococcomyces -related fungal clones were present.